8PKS
Low resolution crystal structure of Keap1-456, a repeat fragment from the human Keap1 beta-propeller
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-20 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000030 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.846, 220.962, 248.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.540 - 2.600 |
R-factor | 0.2161 |
Rwork | 0.214 |
R-free | 0.25910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.814 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.540 | 2.760 |
High resolution limit [Å] | 2.600 | 2.700 |
Rmerge | 0.075 | 0.773 |
Rmeas | 0.080 | 0.815 |
Rpim | 0.026 | 0.258 |
Total number of observations | 44335 | |
Number of reflections | 81023 | 4486 |
<I/σ(I)> | 23.1 | 3.2 |
Completeness [%] | 100.0 | |
Redundancy | 9.3 | 9.9 |
CC(1/2) | 0.999 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | 0.1 M Bis-Tris pH 5.5, 2 M Ammonium sulfate |