8PKC
Structure of Api m1 in complex with the AM1-4 nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-03 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.600, 93.600, 189.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.800 - 4.100 |
| R-factor | 0.262 |
| Rwork | 0.258 |
| R-free | 0.29570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.802 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 4.247 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.118 | 0.910 |
| Number of reflections | 7086 | 687 |
| <I/σ(I)> | 16.12 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.6 | 4.1 |
| CC(1/2) | 0.999 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1M sodium citrate pH 5.5, 15% w/v PEG 6000 |
