8PJH
Crystal structure of human insulin desB30 precursor with an Aspartate-Glycine-Lysine C-peptide in dimer (T2) conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-02-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | I 21 3 |
Unit cell lengths | 78.100, 78.100, 78.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.260 - 1.500 |
R-factor | 0.1723 |
Rwork | 0.172 |
R-free | 0.18160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.141 |
Data reduction software | HKL-2000 (0.93) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.048 | 0.768 |
Number of reflections | 72811 | 1250 |
<I/σ(I)> | 45.7 | 2.2 |
Completeness [%] | 99.2 | 100 |
Redundancy | 5.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | TBD |