8PJ4
Structure of human 48S translation initiation complex after eIF5 release (48S-4)
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Software | Gautomatch,RELION,Coot,EPU,Gctf,UCSF ChimeraX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |