8PJ3
Structure of human 48S translation initiation complex upon transfer of initiator tRNA to eIF5B (48S-3)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Software | Gautomatch,RELION,EPU,Gctf,UCSF ChimeraX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
