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8PHY

crystal structure of powdery mildews Blumeria graminis f. sp. tritici AVRPM2 (2)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2022-02-06
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.9999
Spacegroup nameP 1 21 1
Unit cell lengths36.670, 61.850, 37.160
Unit cell angles90.00, 94.14, 90.00
Refinement procedure
Resolution37.060 - 1.060
R-factor0.1766
Rwork0.175
R-free0.19720
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.449
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (PHENIX)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]37.0601.100
High resolution limit [Å]1.0601.060
Number of reflections687743431
<I/σ(I)>10.64
Completeness [%]93.0
Redundancy10.6
CC(1/2)0.9990.198
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP293.1520 % w/v Polyethylene glycol 3,350; 100 mM BIS-TRIS propane pH 7.5; 200 mM sodium acetate

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PDB entries from 2024-11-06

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