8PHY
crystal structure of powdery mildews Blumeria graminis f. sp. tritici AVRPM2 (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.670, 61.850, 37.160 |
Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
Resolution | 37.060 - 1.060 |
R-factor | 0.1766 |
Rwork | 0.175 |
R-free | 0.19720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.449 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (PHENIX) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.060 | 1.100 |
High resolution limit [Å] | 1.060 | 1.060 |
Number of reflections | 68774 | 3431 |
<I/σ(I)> | 10.64 | |
Completeness [%] | 93.0 | |
Redundancy | 10.6 | |
CC(1/2) | 0.999 | 0.198 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20 % w/v Polyethylene glycol 3,350; 100 mM BIS-TRIS propane pH 7.5; 200 mM sodium acetate |