8PHD
Structure of Human Cdc123 bound to domain 3 of eIF2 gamma and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.462, 126.020, 74.644 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 64.220 - 2.080 |
R-factor | 0.1788 |
Rwork | 0.176 |
R-free | 0.20480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.441 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.220 | 2.370 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmeas | 0.151 | |
Number of reflections | 39743 | 1988 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 93.0 | |
Redundancy | 6.9 | |
CC(1/2) | 0.995 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG3350 0.2 M lithium citrate 1 mM ATP 5 mM MgCl2 |