8PFX
X-ray structure of the adduct formed upon reaction of Lysozyme with K2[Ru2(D-p-FPhF)(CO3)3] in condition B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.720, 76.720, 38.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.360 - 1.030 |
Rwork | 0.192 |
R-free | 0.21440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.933 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.360 | 1.040 |
High resolution limit [Å] | 1.030 | 1.030 |
Rmerge | 0.034 | 0.753 |
Number of reflections | 57486 | 2835 |
<I/σ(I)> | 33.7 | 2.3 |
Completeness [%] | 100.0 | |
Redundancy | 11.4 | |
CC(1/2) | 1.000 | 0.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2 M sodium formate and 0.1 M Hepes, pH 7.5 |