8PFH
Crystal structure of the yeast septin complex Shs1-Cdc12-Cdc3-Cdc10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.885602 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 258.178, 69.373, 92.533 |
| Unit cell angles | 90.00, 109.68, 90.00 |
Refinement procedure
| Resolution | 121.550 - 3.240 |
| R-factor | 0.2826 |
| Rwork | 0.282 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.519 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.550 | 3.650 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rpim | 0.334 | 0.877 |
| Number of reflections | 15695 | 786 |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 63.2 | 10.7 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.937 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20 % PEG 5000, 0.3 M ammonium sulphate, 0.1 M BIS-TRIS pH 6.5 |
