8PFC

Crystal structure of binary complex between Aster yellows witches'-broom phytoplasma effector SAP05 and the zinc finger domain of SPL5 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	78.686, 165.019, 80.862
Unit cell angles	90.00, 109.65, 90.00

Refinement procedure

Resolution	74.210 - 2.200
R-factor	0.22438
Rwork	0.223
R-free	0.25069
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.509
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.510	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.129	2.104
Rmeas	0.134	2.182
Rpim	0.035	0.577
Total number of observations	1386043
Number of reflections	98262	4890
<I/σ(I)>	10.4
Completeness [%]	100.0	100
Redundancy	14.1	14.3
CC(1/2)	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	NULL