8PEI
Crystal structure of the biphotochromic fluorescent protein SAASoti (C21N/V127T variant) in its green on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-11 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.583, 104.583, 151.187 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.850 - 3.000 |
| R-factor | 0.29356 |
| Rwork | 0.291 |
| R-free | 0.34012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.054 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.280 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.705 | 4.746 |
| Rmeas | 0.719 | 4.830 |
| Rpim | 0.135 | 0.884 |
| Total number of observations | 273101 | 43605 |
| Number of reflections | 10148 | 1516 |
| <I/σ(I)> | 7.1 | 1.1 |
| Completeness [%] | 98.0 | |
| Redundancy | 26.9 | 28.8 |
| CC(1/2) | 0.985 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.1 M Tris pH 8.0; 30% w/v Polyethylene glycol monomethyl ether 2,000 |
