8PE8
Symmetry expanded D7 local refined map of mitochondrial heat-shock protein 60-like protein from Chaetomium thermophilum
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Phase Plate | OTHER |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.46 |
| Number of Particle | 287314 |
| Software | cryoSPARC,Gctf,Coot,EPU,PHENIX |
| Symmetry | D7 |
| CTF correction | PHASE FLIPPING ONLY |
