8PE2
Crystal structure of Gel4 in complex with Nanobody 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.992, 96.992, 106.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.540 - 2.050 |
| R-factor | 0.17369 |
| Rwork | 0.172 |
| R-free | 0.20603 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w62 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.784 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.142 | |
| Number of reflections | 36838 | 5292 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.996 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | NPS, buffer system 3, P500MME P20K |
