8PCH
CRYSTAL STRUCTURE OF PORCINE CATHEPSIN H DETERMINED AT 2.1 ANGSTROM RESOLUTION: LOCATION OF THE MINI-CHAIN C-TERMINAL CARBOXYL GROUP DEFINES CATHEPSIN H AMINOPEPTIDASE FUNCTION
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.442, 68.730, 86.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.198 * |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2act |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.530 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MAIN |
Refinement software | MAIN |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 * |
High resolution limit [Å] | 2.100 * |
Rmerge | 0.110 * |
Total number of observations | 102384 * |
Number of reflections | 15946 |
Completeness [%] | 98.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 * | PROTEIN WAS CRYSTALLIZED USING SITTING DROP VAPOR DIFFUSION METHOD FROM 0.05M NA-ACETATE AND 5% MME PEG 5K BUFFER, PH 4.8. CONCENTRATION OF APPLIED PROTEIN WAS 11 MG/ML., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | drop | sodium acetate | 20 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | reservoir | sodium acetate | 0.05 (M) | |
5 | 1 | reservoir | mPEG5000 | 5 (%) |