8PC1
Sub-tomogram average of the closed conformation of the Nap adhesion complex from the human pathogen Mycoplasma genitalium.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum SE |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SUBTOMOGRAM AVERAGING |
Resolution (Å) | 18 |
Software | ISOLDE,cryoSPARC |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |