8PB8
PsiM in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-01 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 43 |
Unit cell lengths | 62.200, 62.200, 154.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.710 - 2.531 |
R-factor | 0.2007 |
Rwork | 0.198 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.649 |
Data reduction software | XDS (20220110) |
Data scaling software | XDS (20220110) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.710 | 2.622 |
High resolution limit [Å] | 2.531 | 2.531 |
Rmerge | 0.350 | 2.197 |
Rmeas | 0.383 | 2.402 |
Rpim | 0.154 | 0.965 |
Number of reflections | 19419 | 1786 |
<I/σ(I)> | 5.38 | 0.81 |
Completeness [%] | 99.1 | 91.68 |
Redundancy | 6 | 6 |
CC(1/2) | 0.977 | 0.249 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 100 mM bis-Tris propane pH 7.0, 30% PEG 300, 15% PEG 1000 |