8PAY
Structure of the E.coli DNA polymerase sliding clamp with a covalently bound peptide 2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-09 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.858, 66.537, 80.798 |
Unit cell angles | 90.00, 128.52, 90.00 |
Refinement procedure
Resolution | 52.610 - 1.210 |
R-factor | 0.1517 |
Rwork | 0.150 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.172 |
Data scaling software | XDS |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.610 | 1.310 |
High resolution limit [Å] | 1.210 | 1.210 |
Number of reflections | 110085 | 5493 |
<I/σ(I)> | 21 | |
Completeness [%] | 96.0 | |
Redundancy | 13.6 | |
CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293.15 | 0.2 M Sodium fluoride 20% PEG 3350, pH 7.3 |