8PAQ
Structure of the small subunit of the laccase-like Nlac protein from Pleurotus eryngii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979257 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.720, 124.720, 55.812 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.160 - 1.600 |
R-factor | 0.1949 |
Rwork | 0.194 |
R-free | 0.21910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.780 |
Data reduction software | autoPROC |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 58835 | 9324 |
<I/σ(I)> | 14.29 | 0.69 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 26.2 | |
CC(1/2) | 0.999 | 0.385 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1 M Imidazole at pH 8.0 and 2.5 M NaCl |