8PAG

Crystal structure of the ectodomain of Norway rat pestivirus E2 glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	90
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS 12M
Wavelength(s)	2.7552
Spacegroup name	P 43 2 2
Unit cell lengths	151.920, 151.920, 59.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.040 - 3.500
R-factor	0.2697
Rwork	0.269
R-free	0.28270
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.168
Data reduction software	xia2
Data scaling software	xia2
Phasing software	HKL2Map
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.040	3.625
High resolution limit [Å]	3.500	3.500
Rmerge	0.169	5.688
Rmeas	0.170	5.729
Rpim	0.021	0.663
Number of reflections	8374	684
<I/σ(I)>	23.79	0.97
Completeness [%]	89.8	75.66
Redundancy	70	72.2
CC(1/2)	1.000	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.2 M sodium formate, 20% PEG 3350