8P9V

Crystal Structure of Two-Domain Laccase mutant M199G/R240H/D268N from Streptomyces griseoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	120
Detector technology	PIXEL
Collection date	2022-12-15
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	74.370, 93.920, 119.591
Unit cell angles	90.00, 91.10, 90.00

Refinement procedure

Resolution	23.760 - 2.200
R-factor	0.2171
Rwork	0.215
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.989
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER (2.8.2 (svn 8338))
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.760	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	82258	8233
<I/σ(I)>	4.89	1.35
Completeness [%]	98.4	96.66
Redundancy	4.6	4.7
CC(1/2)	0.960	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	296	15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate condition #31 of BCS-1 from Molecular Dimensions, UK