8P9R
Structure of the periplasmic domain of ExbD from E. coli in complex with TonB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.3799 |
Spacegroup name | P 41 |
Unit cell lengths | 49.360, 49.360, 63.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.640 - 1.520 |
R-factor | 0.2115 |
Rwork | 0.210 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.048 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.070 | 1.610 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmeas | 0.061 | 0.816 |
Number of reflections | 46421 | 7485 |
<I/σ(I)> | 21.45 | 2.45 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13.3 | |
CC(1/2) | 0.990 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 10 %w/v Glycerol, 3M ammonium sulfate |