8P9E

Crystal structure of wild type p63-p73 heterotetramer (tetramerisation domain) in complex with darpin 1810 F11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	84.704, 60.728, 53.866
Unit cell angles	90.00, 100.78, 90.00

Refinement procedure

Resolution	49.050 - 2.250
R-factor	0.19226
Rwork	0.189
R-free	0.25012
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.277
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.099	0.777
Rmeas	0.113	0.905
Rpim	0.042	0.344
Number of reflections	12746	1208
<I/σ(I)>	8.6	2.1
Completeness [%]	99.4
Redundancy	7	6.8
CC(1/2)	0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.2M sodium chloride, 25% PEG3350, 0.1M bis-tris pH 6.5