8P91
Hfq from Chromobacterium haemolyticum; a P6 space group monomer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-23 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 6 |
Unit cell lengths | 65.145, 65.145, 27.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.320 - 1.400 |
R-factor | 0.169 |
Rwork | 0.155 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.909 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.320 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.095 | 0.730 |
Rmeas | 0.106 | 0.856 |
Rpim | 0.045 | 0.442 |
Total number of observations | 68788 | 2424 |
Number of reflections | 13426 | 663 |
<I/σ(I)> | 10.7 | 1.6 |
Completeness [%] | 99.9 | |
Redundancy | 5.1 | 3.7 |
CC(1/2) | 0.996 | 0.478 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | #23 of JBScreen Nuc-Pro 1 (Jena Bioscience) 15% PEG4000, 50 mM Tris-HCl, pH 7.5, 150 mM KCl, 20 mM MgCl2 |