8P8S

Recombinant Ym2 crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	51.090, 59.900, 59.880
Unit cell angles	90.00, 94.14, 90.00

Refinement procedure

Resolution	37.450 - 1.170
R-factor	0.158
Rwork	0.158
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS (VERSION Mar 15, 2019 BUILT=20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.730	1.240
High resolution limit [Å]	1.170	1.170
Rmeas	0.080	0.358
Number of reflections	119183	18719
<I/σ(I)>	15.12	4.74
Completeness [%]	98.4	95.9
Redundancy	6.76
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M sodium acetate pH 6.0 and 200 mM CaCl