8P8R
Ex vivo Ym1 crystal structure
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 50.518, 60.248, 60.116 |
| 格子定数 [度] | 90.00, 94.48, 90.00 |
精密化法
| 分解能 | 26.910 - 1.420 |
| R因子 | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.980 |
| Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180808) |
| Data scaling software | Aimless (v. 0.7.4) |
| Phasing software | PHASER (v. 2.8.2) |
| Refinement software | BUSTER (2.10.3) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 59.900 | 1.500 |
| 分解能 [Å] (高) | 1.420 | 1.420 |
| Rmeas | 0.055 | 1.382 |
| 独立反射数 | 66956 | 10672 |
| <I/σ(I)> | 7.2 | 1.19 |
| 完全性 [%] | 98.1 | |
| 冗長性 | 3.33 | |
| CC(1/2) | 0.998 | 0.395 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | IN CELL | 310 | Spontaneous in vivo crystallisation. Single ex vivo Ym1 crystals were harvested from the BAL fluid of mev/mev mice using mounted cryo-loops. |
