8P6K
Structure of the hypervariable region of Streptococcus pyogenes M3 protein
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.474, 24.107, 78.007 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 75.247 - 1.918 |
| Rwork | 0.230 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.250 | 1.950 |
| High resolution limit [Å] | 1.918 | 1.920 |
| Rmeas | 0.058 | 1.741 |
| Number of reflections | 21415 | 1045 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.900 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40% MPD |
