8P6G
Crystal structure of the improved version of the Genetically Encoded Green Calcium Indicator YTnC2-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 65.736, 65.736, 315.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.140 - 1.950 |
| R-factor | 0.1857 |
| Rwork | 0.183 |
| R-free | 0.22883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.029 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.140 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.313 | 3.567 |
| Rmeas | 0.321 | 3.663 |
| Rpim | 0.071 | 0.830 |
| Total number of observations | 1130475 | 76035 |
| Number of reflections | 55880 | 3979 |
| <I/σ(I)> | 6.5 | 0.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.2 | 19.1 |
| CC(1/2) | 0.992 | 0.468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2M Sodium fluoride, 0.1M Bis-tris propane, 20% PEG 3350 pH 7.5 |
