8P5S
Crystal structure of the homohexameric 2-oxoglutarate dehydrogenase OdhA from Corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-26 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | H 3 2 |
Unit cell lengths | 150.987, 150.987, 314.335 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.530 - 2.459 |
R-factor | 0.2061 |
Rwork | 0.204 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8p5r |
RMSD bond length | 0.007 |
RMSD bond angle | 0.860 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.520 | 2.700 |
High resolution limit [Å] | 2.460 | 2.460 |
Rpim | 0.047 | 0.466 |
Number of reflections | 38261 | 1913 |
<I/σ(I)> | 15.5 | 1.6 |
Completeness [%] | 94.4 | 63.8 |
Redundancy | 10.4 | |
CC(1/2) | 0.998 | 0.658 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Hepes-NaOH pH 7.5, 5% (w/v) PEG 4000, 30% (v/v) methylpentanediol (MPD) |