8P4Y
Coiled-coil protein origami triangle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.840, 36.980, 44.670 |
| Unit cell angles | 97.25, 105.76, 95.45 |
Refinement procedure
| Resolution | 36.347 - 2.052 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.747 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.350 | 2.126 |
| High resolution limit [Å] | 2.050 | 2.052 |
| Rmeas | 0.039 | 0.930 |
| Number of reflections | 12496 | 1233 |
| <I/σ(I)> | 15.08 | 1.32 |
| Completeness [%] | 97.5 | 96.18 |
| Redundancy | 3.62 | 3.75 |
| CC(1/2) | 0.990 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 50% PEG 400, 100 mM CHES/Sodiumhydroxide pH 9.5, 200 mM sodium chloride |
