8P4R
In situ structure average of GroEL14-GroES14 complexes in Escherichia coli cytosol obtained by cryo electron tomography
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SUBTOMOGRAM AVERAGING |
Resolution (Å) | 11.9 |
Software | RELION,STOPGAP,SerialEM |
Symmetry | D7 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |