8P2P

Polymeric form of the BTB domain of human ZBTB18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	I 41
Unit cell lengths	188.908, 188.908, 139.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.360 - 4.150
R-factor	0.3458
Rwork	0.345
R-free	0.35990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.360	4.220
High resolution limit [Å]	4.150	4.150
Rpim	0.041	0.472
Number of reflections	18588	886
<I/σ(I)>	8.9	1.4
Completeness [%]	100.0	100
Redundancy	14	14.2
CC(1/2)	0.998	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	MgCl2, EtOH, imidazole