8P1Z
Arabidopsis thaliana cytosolic seryl-tRNA synthetase in addition of dithiothreitol (DTT)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 97.410, 45.889, 107.068 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 19.158 - 2.300 |
| Rwork | 0.226 |
| R-free | 0.28880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.461 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.167 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.816 |
| Number of reflections | 20826 | 2088 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 97.8 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Magnesium chloride, PEG 4000, TRIS pH 7 |
