8P10
The crystal structure of the C-terminal domain of Mengla nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.309, 85.710, 146.241 |
| Unit cell angles | 88.52, 76.45, 80.26 |
Refinement procedure
| Resolution | 47.990 - 3.260 |
| R-factor | 0.2758 |
| Rwork | 0.274 |
| R-free | 0.30460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (V.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.156 | 4.052 |
| High resolution limit [Å] | 3.250 | 3.256 |
| Rmerge | 0.103 | 0.713 |
| Number of reflections | 30881 | 6177 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 89.2 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.998 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Ammonium sulfate, 0.1M MES pH 6.5, 30% PEG5000MME |
