8P0U
Thogoto virus polymerase in Mode B conformation with defined endonuclease domain and bound to 32-mer loop promoter RNA
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.92 |
| CTF correction | PHASE FLIPPING ONLY |
