8P0C

Rubella virus p150 macro domain (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	I 2 2 2
Unit cell lengths	72.682, 87.569, 135.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 - 1.720
R-factor	0.1883
Rwork	0.186
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.818
Data reduction software	autoPROC (1.0.5)
Data scaling software	STARANISO (1.0.4)
Phasing software	MrBUMP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.100	1.949
High resolution limit [Å]	1.720	1.723
Rmerge	0.112	1.030
Rpim	0.052	0.514
Number of reflections	23298	1165
<I/σ(I)>	9.9	1.6
Completeness [%]	91.3	53.5
Redundancy	5.3	4.6
CC(1/2)	0.997	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	291	14.4% PEG 6K, 1.26 M LiCl, 0.1 M Bicine pH 9, supplemented with 10% glycerol for prior to freezing