8OZU
Fucosidase crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 308.100, 81.169, 194.237 |
| Unit cell angles | 90.00, 123.88, 90.00 |
Refinement procedure
| Resolution | 148.634 - 2.250 |
| Rwork | 0.193 |
| R-free | 0.22390 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.741 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 161.270 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.103 | |
| Rmeas | 0.119 | |
| Rpim | 0.058 | 0.492 |
| Number of reflections | 189127 | 27416 |
| <I/σ(I)> | 10.1 | 1.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 4 | |
| CC(1/2) | 0.996 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20.00 % w/v Polyethylene glycol 4,000 10.00 % w/v Glycerol 200 mM Magnesium sulfate |
