8OZB

Crystal structure of Nup35-Nb complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-05
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9763
Spacegroup name	P 2 2 21
Unit cell lengths	49.120, 77.220, 127.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.090
R-factor	0.24306
Rwork	0.242
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.675
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.020	2.150
High resolution limit [Å]	2.090	2.090
Number of reflections	29113	1993
<I/σ(I)>	8.1
Completeness [%]	98.6
Redundancy	12.5
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05 M HEPES (pH 6.5); 25% v/v PEG400; 0.05 M NaCl, 0.01 M MgCl2.