8OXL

crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2022-06-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	57.552, 57.552, 121.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.820 - 1.560
R-factor	0.1921
Rwork	0.191
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.435
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.820	1.650
High resolution limit [Å]	1.560	1.560
Number of reflections	29943	8514
<I/σ(I)>	8.18
Completeness [%]	97.7
Redundancy	3.3
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic pH 8.2