8OXK
crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.941, 28.955, 29.175 |
Unit cell angles | 90.00, 96.67, 90.00 |
Refinement procedure
Resolution | 31.270 - 1.370 |
R-factor | 0.1691 |
Rwork | 0.168 |
R-free | 0.18590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.640 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.270 | 1.470 |
High resolution limit [Å] | 1.370 | 1.380 |
Number of reflections | 21484 | 1177 |
<I/σ(I)> | 12.25 | |
Completeness [%] | 79.3 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.516 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.16 M Calcium acetate hydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5, 14.4% w/v Polyethylene glycol 8,000 |