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8OXK

crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA10

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron sitePETRA III, EMBL c/o DESY
BeamlineP14 (MX2)
Temperature [K]100
Detector technologyPIXEL
Collection date2022-08-20
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.9763
Spacegroup nameC 1 2 1
Unit cell lengths125.941, 28.955, 29.175
Unit cell angles90.00, 96.67, 90.00
Refinement procedure
Resolution31.270 - 1.370
R-factor0.1691
Rwork0.168
R-free0.18590
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.021
RMSD bond angle1.640
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.19.2_4158)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]31.2701.470
High resolution limit [Å]1.3701.380
Number of reflections214841177
<I/σ(I)>12.25
Completeness [%]79.3
Redundancy6.9
CC(1/2)0.9990.516
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP293.150.16 M Calcium acetate hydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5, 14.4% w/v Polyethylene glycol 8,000

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