8OXI
crystal structure of powdery mildews Blumeria graminis f. sp. tritici AVRPM2(1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.405, 53.864, 61.813 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.800 - 1.510 |
R-factor | 0.1944 |
Rwork | 0.194 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.216 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.800 | 1.550 |
High resolution limit [Å] | 1.510 | 1.510 |
Number of reflections | 24155 | 2348 |
<I/σ(I)> | 7.72 | |
Completeness [%] | 99.7 | |
Redundancy | 6.9 | |
CC(1/2) | 0.997 | 0.260 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M BIS-TRIS pH 6.5, 28% w/v Polyethylene glycol monomethyl ether 2,000 |