8OXH
crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 62.451, 62.451, 202.322 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.190 - 2.500 |
R-factor | 0.266 |
Rwork | 0.263 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.21rc1_4933) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.200 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 8696 | 1350 |
<I/σ(I)> | 24.36 | |
Completeness [%] | 99.9 | |
Redundancy | 18.3 | |
CC(1/2) | 1.000 | 0.402 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20 % w/v Polyethylene glycol 3 350, 200 mM Sodium fluoride |