8OX3

Structure of the murine LYVE-1 (lymphatic vessel endothelial receptor-1) hyaluronan binding domain bound with octasaccharide hyaluronan.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	C 1 2 1
Unit cell lengths	86.107, 56.963, 30.908
Unit cell angles	90.00, 106.27, 90.00

Refinement procedure

Resolution	46.900 - 1.050
R-factor	0.1512
Rwork	0.151
R-free	0.16290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.189
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.21rc1_4899)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	1.070
High resolution limit [Å]	1.050	1.050
Rmeas	0.101	1.075
Number of reflections	46097	193
<I/σ(I)>	11.1
Completeness [%]	83.0
Redundancy	6.4
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG5000, 0.1M bis-Tris pH6.5, 18 mg/ml