8OWY

Crystal structure of METTL6 mutant 40-269 bound to SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.008
Spacegroup name	C 1 2 1
Unit cell lengths	81.971, 138.129, 39.660
Unit cell angles	90.00, 115.26, 90.00

Refinement procedure

Resolution	39.110 - 3.200
R-factor	0.2619
Rwork	0.260
R-free	0.29960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.041
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.110	3.315
High resolution limit [Å]	3.200	3.200
Rmerge	0.205	2.196
Number of reflections	6561	318
<I/σ(I)>	6.97
Completeness [%]	85.3
Redundancy	6.5
CC(1/2)	0.995	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M BisTRIS, 0.2 M ammonium sulfate and 25% PEG3350