8OWI
Crystal structure of the corona-targeting domain of CENP-E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-20 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 84.018, 84.018, 65.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.420 - 2.140 |
R-factor | 0.2176 |
Rwork | 0.216 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.574 |
Data scaling software | STARANISO |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.420 | 2.340 |
High resolution limit [Å] | 2.140 | 2.140 |
Rpim | 0.026 | 0.563 |
Number of reflections | 9178 | 82 |
<I/σ(I)> | 17.9 | 1.3 |
Completeness [%] | 68.8 | 15 |
Redundancy | 24.8 | |
CC(1/2) | 1.000 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Potassium citrate tribasic monohydrate pH 8.3, 20% w/v polyethene glycol 3,350, with 30% glycerol (cryoprotectant). |