8OVP
X-ray structure of the iAspSnFR in complex with L-aspartate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-17 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.310, 105.880, 77.280 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 38.600 - 1.700 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.039 | 0.548 |
| Number of reflections | 116407 | 18188 |
| <I/σ(I)> | 16.7 | 2.3 |
| Completeness [%] | 98.1 | 97.5 |
| Redundancy | 6.3 | 6.4 |
| CC(1/2) | 0.999 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M magnesium acetate, 18% (m/v) PEG 3350, 40 mM L-aspartate |
