8OUM

Arf GTPase from the asgard Gerdarchaea : GerdArfR1 bound to GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980112
Spacegroup name	P 1 21 1
Unit cell lengths	87.250, 54.880, 121.790
Unit cell angles	90.00, 94.47, 90.00

Refinement procedure

Resolution	46.410 - 2.670
R-factor	0.2376
Rwork	0.236
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	BUSTER (2.10.4 (20-OCT-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.830
High resolution limit [Å]	2.670	2.670
Rmeas	0.034	0.260
Number of reflections	32436	4627
<I/σ(I)>	6
Completeness [%]	97.8
Redundancy	6.8
CC(1/2)	0.990	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	8.5% PEG 8000, 0.1M MgCl2 and 0.1M Tris pH 7.0