8OUM
Arf GTPase from the asgard Gerdarchaea : GerdArfR1 bound to GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980112 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.250, 54.880, 121.790 |
| Unit cell angles | 90.00, 94.47, 90.00 |
Refinement procedure
| Resolution | 46.410 - 2.670 |
| R-factor | 0.2376 |
| Rwork | 0.236 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.830 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmeas | 0.034 | 0.260 |
| Number of reflections | 32436 | 4627 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 97.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.446 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 8.5% PEG 8000, 0.1M MgCl2 and 0.1M Tris pH 7.0 |
