8OUK
Arf GTPase from the asgard Hodarchaea : HodArfR1 bound to GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979415 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.120, 59.120, 119.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.720 - 1.800 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.044 | 0.582 |
| Number of reflections | 20387 | 1982 |
| <I/σ(I)> | 39.8 | |
| Completeness [%] | 99.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.981 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 2.0 M Sodium formate and 0.1M HEPES pH 7.5 |
