8OUH
Complex of human ASCT2 with Syncytin-1
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | TFS Selectris X |
Phase Plate | VOLTA PHASE PLATE |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.62 |
Number of Particle | 4018074 |
Software | PHENIX,cryoSPARC,EPU,Coot,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |