8OU7
Cereblon isoform 4 in complex with novel Benzamide-Type Cereblon Binder 11d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-17 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.409, 59.548, 88.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.554 - 1.700 |
Rwork | 0.176 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.809 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.554 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 33503 | 5334 |
<I/σ(I)> | 14.74 | |
Completeness [%] | 100.0 | |
Redundancy | 12.85 | |
CC(1/2) | 0.999 | 0.619 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | 0.5 M (NH4)H2PO4 |