8OU4
Cereblon isoform 4 in complex with novel Benzamide-Type Cereblon Binder 11a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-17 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.511, 59.542, 88.185 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.580 - 2.250 |
Rwork | 0.222 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.758 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.580 | 2.331 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 14648 | 2311 |
<I/σ(I)> | 10.68 | |
Completeness [%] | 99.8 | |
Redundancy | 12.9 | |
CC(1/2) | 0.998 | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | 0.5 M (NH4)H2PO4 |